How a DNA polymerase clamp loader opens a sliding clamp

How a DNA polymerase clamp loader opens a sliding clamp

THE J B C © 2001 by The American Society for Biochemistry

Interplay of Clamp Loader Subunits in Opening the Sliding Clamp of Escherichia coli DNA Polymerase III Holoenzyme* Received for publication, July 18, 2001, and in revised form, September 21, 2001 Published, JBC Papers in Press, September 25, 2001, DOI 10.1074/jbc.M106780200 Frank P. Leu‡ and Mike O'Donnell§¶

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How a DNA Polymerase Clamp Loader Opens a Sliding Clamp

Dec 23, 2011 · Biochemical data demonstrate that ATP binding also enables the clamp loader to bind to and open the sliding clamp and that the binding of DNA triggers ATP hydrolysis and release of the closed clamp on DNA (21, 22). A molecular understanding of the mechanism that integrates clamp opening with its loading onto DNA is lacking because none of the structures …

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Clamp loader ATPases and the evolution of DNA replication

Apr 20, 2012 · Clamp loader interactions to open the sliding clamp. (a) The clamp is held in a distorted conformation. In the top panel, displacement vectors compared from the closed planar structure (PDB code 1CZD) to that of the open form (PDB code 3U60) are mapped out onto the structure and are scaled up by a factor of 4 and color coded blue to red. In the

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Molecular Biology Quiz 5 Flashcards | Quizlet

DNA polymerase can only synthesize in the 5' to 3' direction- attaches deoxyribonucleoside triphosphate via open 3' OH group. Clamp Loader. chaperones the sliding clamp until it reaches the primer-template junction. Then it also unloads the sliding clamp when replication is finished.

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Cryo-EM structures reveal high-resolution mechanism of a

Sep 23, 2021 · 31 shaped sliding clamp proteins that wrap around DNA to activate 32 DNA polymerases and other factors (Moldovan et al., 2007). 33 Sliding clamps are regulated by their presence on DNA, which in 34 turn is governed by clamp loaders that open the sliding clamp ring 35 and place it onto DNA (Kelch, 2016). The clamp loader of

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How a DNA polymerase clamp loader opens a sliding clamp

We present structures for the ATP-bound state of the clamp loader complex from bacteriophage T4, bound to an open clamp and primer-template DNA. The clamp loader traps a spiral conformation of the open clamp so that both the loader and the clamp match the helical symmetry of DNA. One structure reveals that ATP has been hydrolyzed in one subunit and …

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RCSB PDB - 3U60: Structure of T4 Bacteriophage Clamp

Oct 11, 2011 · Processive chromosomal replication relies on sliding DNA clamps, which are loaded onto DNA by pentameric clamp loader complexes belonging to the AAA+ family of adenosine triphosphatases (ATPases). We present structures for the ATP-bound state of the clamp loader complex from bacteriophage T4, bound to an open clamp and primer-template …

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DNA Polymerase Clamp Loaders - UMass Med

Fig. 1. Clamp loaders and sliding clamps. ( A) Clamp-loading reaction. The clamp loader has low affinity for both clamp and primer-template DNA in the absence of ATP. Upon binding ATP, the clamp loader can bind the clamp and open it. The binding of primer-t emplate DNA activates ATP hydrolysis, leading to ejection of the clamp loader.

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DNA clamp - Wikipedia

Dec 23, 2011 · Biochemical data demonstrate that ATP binding also enables the clamp loader to bind to and open the sliding clamp and that the binding of DNA triggers ATP hydrolysis and release of the closed clamp

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Cryo-EM structures reveal high-resolution mechanism of a

Sep 23, 2021 · Sliding clamps are ring-shaped protein complexes that are integral to the DNA replication machinery of all life. Sliding clamps are opened and installed onto DNA by clamp loader AAA+ ATPase complexes. However, how a clamp loader opens and closes the sliding clamp around DNA is still unknown. Here, we describe structures of the S. cerevisiae clamp …

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Molecular Biology - Chapter 5: DNA Replication Flashcards

Dec 01, 2011 · Instead, ATP-dependent complexes known as clamp loaders open the sliding clamps and load them on to primed DNA in the correct orientation for productive engagement of the polymerase (the clamp loaders are the γ/τ complex in bacteria, Replication Factor-C (RFC) in eukaryotes and archaea, and gp44/62 in T4 bacteriophage (Fig. 1B)).

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CiteSeerX — Citation Query C-terminal domain

Chromosomal DNA replication is dependent on processive DNA synthesis. Across the three domains of life and in certain viruses, a toroidal sliding clamp confers processivity to replicative DNA polymerases by encircling the DNA and engaging the polymerase in …

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DNA Polymerase Clamp Loaders - UMass Med

DNA Polymerase Clamp Loaders. Chromosomal DNA replication requires that DNA polymerases be tethered to ring-shaped sliding clamps that encircle the DNA and allow for high-speed, processive replication. Sliding clamps are loaded onto DNA by the clamp loader complex, a pentameric assembly of proteins of the AAA+ family of ATPases.

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DNA Replication: How Does a Sliding Clamp Slide

The clamp loader traps a spiral conformation of the open clamp so that both the loader and the clamp match the helical symmetry of DNA. One structure reveals that ATP has been hydrolyzed in one subunit, and suggests that clamp closure and ejection of the loader involves disruption of the ATP-dependent match in symmetry.

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Clamp Loader ATPases and the Evolution of DNA Replication

Clamp loader interactions to open the sliding clamp. (a) The clamp is held in a distorted conformation. In the top panel, displacement vectors compared from the closed planar structure (PDB code 1CZD) [13] to that of the open form (PDB code 3U60) [60] are mapped out onto the structure and are scaled up by a factor of 4 and color coded blue to red.

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How a DNA Polymerase Clamp Loader Opens a Sliding Clamp

How a DNA Polymerase Clamp Loader Opens a Sliding Clamp

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Allosteric communication in DNA polymerase clamp loaders

Apr 13, 2021 · Sliding DNA clamps and the ATP-driven clamp-loader complexes that load them onto DNA are essential components of the DNA replication machinery in all branches of life (Yao and O'Donnell, 2016).Sliding clamps, such as proliferating cell nuclear antigen (PCNA) in eukaryotes, are ring-shaped proteins that enable highly processive DNA replication by tethering …

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DNA polymerase clamp loaders and DNA recognition

Feb 07, 2005 · Sliding clamps require active placement on DNA for rapid DNA synthesis. This task is carried out by ATP-dependent clamp loader complexes, which stabilize an opening of the clamp transiently so as to allow placement on DNA (Fig. 1; ).The interaction between clamp loader and clamp takes place only when the loader is bound to ATP .The recognition by the clamp …

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Molecular Biology - Chapter 5: DNA Replication Flashcards

The loader opens due to ATP binding to it and the sliding clamp opens. Step 2 of sliding clamp process. ATP hydrolysis locks sliding clamp around DNA and releases the clamp loader. Step 3 of sliding clamp process. Clamp closes and complex dissociates, and DNA pol binds to the sliding clamp. why cant dna polymerase fill in the gaps after

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Molecular Biology Quiz 5 Flashcards | Quizlet

DNA polymerase can only synthesize in the 5' to 3' direction- attaches deoxyribonucleoside triphosphate via open 3' OH group. Clamp Loader. chaperones the sliding clamp until it reaches the primer-template junction. Then it also unloads the sliding clamp when replication is finished.

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